HiPure Fruit RNA Kit

The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A RNA(mRNA) purification, nuclease protection and in vitro translation. Details Specifications Features Specifications Main Functions Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent Applications RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation Purification method Mini spin column Purification technology Silica technology, DNA filtration technology Process method Manual (centrifugation or vacuum) Sample type Simple plant, petal, peel, seed Sample amount 50-150mg Elution volume ≥300μl Time per run ≤15 minutes（1-24 samples） Liquid carrying volume per column 800µl Binding yield of column 100µg Principle The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit. Advantages Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration Fast – several samples can be extracted in 15 minutes by column method Wide range – samples including animals, plants, bacteria, cells, etc High output – large sample amount, and the yield can be up to 200μg Kit Contents Contents R401402 D401403 Purification Times 50 Preps 250 Preps HiPure RNA Mini Columns 50 250 2ml Collection Tubes 50 250 PlantZol Reagent 60 ml 200 ml Buffer GXP 30 ml 150 ml Buffer RW1 50 ml 200 ml Buffer RW2* 20 ml 2 x 50 ml RNase Free Water 10 ml 30 ml Storage and Stability The Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.