Pre-processing of blood sample genomic DNA extraction 96 Samples

Sample Handling: Collect 0.1-1 ml blood sample. Sample Preparation: For blood samples: Add 2-3 times the volume of 1x erythrocyte lysate. Fully invert and mix well. Centrifuge at 12,000 rpm for 1 minute. Carefully remove the supernatant. The precipitate should be white or light red. Add 400 μl reagent buffer I and 10 μl proteinase K (10 mg/ml) to the precipitate. Vortex and mix for 15 seconds, then incubate at room temperature for 2 minutes. Special Consideration for Low-Level Biological Blood: If processing poultry, bird, or other low-level biological blood with nuclei-containing red blood cells and low sample volume: Skip adding 1x erythrocyte lysate. Directly add 200 μl of reagent buffer I (total volume not exceeding 500 μl). Incubate at room temperature for 2 minutes. Digestion: Add 10 μl proteinase K (10 mg/ml) to the mixture. Mix thoroughly by inversion. Digest at 65°C for 10 minutes, inverting and mixing 6-7 times during digestion until complete. Transfer to Reagent Plate: Add the lysate from the previous step to the 96-well reagent plate. Follow specific steps outlined in the automatic extraction protocol for further processing.