HiPure Plasmid EF 96 Kit

Introduction The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water. Details Specifications Features Specifications Main Functions Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate Applications Cell transfection, animal injection, etc. Purification method 96 well plate Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type Conventional plasmid, plasmid less than 30KB Sample amount 1-5ml（x96） Yield 30μg Elution volume ≥75μl Time per run ≤60 minutes Liquid carrying volume per column 800μl Binding yield of column 70μg Principle The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA). Advantages High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc. Fast – silica gel column purification is much faster than ion exchange Ultra low endotoxin – content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, etc. Kit Contents Contents P115701 P115702 P115703 Purification Times 1 x 96 Preps 4 x 96 Preps 20 x 96 Preps RNase A 10 mg 20 mg 100 mg Buffer P1 30 ml 120 ml 550 ml Buffer P2 30 ml 120 ml 550 ml Buffer LEN3 15 ml 60 ml 300 ml Buffer LN4 80 ml 270 ml 2 x 700 ml Buffer LN5 40 ml 220 ml 1100 ml Buffer PW1 40 ml 220 ml 1100 ml Buffer PW2 25 ml 100 ml 2 x 200 ml Elution Buffer 30 ml 60 ml 250 ml Lysate Clear Plate 1 4 20 HiPure DNA Plate 1 4 20 1.6ml Collection Plate 2 8 40 0.5ml Collection Plate 1 4 20 Storage and Stability The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C. Purchase Guide Name CAT NO Sample amount Column type Elution volume Yield Time per run Centrifuge requirements HiPure Plasmid EF Mini Kit P1154 10-15ml 1.5ml column ≥75μl 10–70µg/15ml ≤40 minutes small centrifuge HiPure Plasmid EF 96 Kit P1157 1-5ml 96 well plate ≥75μl 30µg/1ml ≤60 minutes 96 well plate centrifuge HiPure Plasmid EF Midi Kit P1231 30-50ml 15ml column ≥0.4ml 10-500μg/50ml ≤60 minutes 15ml centrifuge HiPure Plasmid EF Maxi Kit P1156 100-200ml 50ml column ≥0.8ml 100–1500µg/200ml ≤60 minutes 50ml centrifuge HiPure Plasmid EF Mega Kit P1116 500ml 50ml column ≥0.8ml 10mg/500ml ≤70 minutes 50ml centrifuge