HiPure Blood RNA Mini Kit

The Kit provides fast purification of high-quality RNA from whole blood, cells, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A RNA (mRNA) purification, nuclease protection, and in vitro translation. Details Specifications Features Specifications Main Functions Isolation of total RNA from 1-1.5ml whole blood Applications qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization, and SNP detection Purification method Mini spin column Purification technology Silica technology, acid phenol/guanidine extraction technology (MagZol pretreatment technology) Process method Manual (centrifugation or vacuum) Sample type Fresh or frozen blood, mammalian blood Sample amount ≤1.5 ml whole blood Yield 2-100μg Elution volume ≥20μl Liquid carrying volume per column 800µl Binding yield of column 100μg Principle The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application. Advantages High quality – high purity total RNA can be directly used in various sensitive downstream applications Fast – isolation of several samples can be completed in 20 minutes by using the column purification method Sensitive – RNA can be purified at the level of PG Kit Contents Contents R416102 R416103 Purification Times 50 Preps 250 Preps HiPure DNA Mini Columns 50 250 HiPure RNA Mini Columns I 50 250 2ml Collection Tubes 100 500 10 x Buffer RBC 50 ml 3 x 100 ml RTL Lysis Buffer 50 ml 250 ml Buffer RW1 50 ml 250 ml Buffer RW2* 20 ml 2 x 50 ml RNase Free Water 10 ml 30 ml Storage and Stability HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.