HiPure HP Plant RNA Mini Kit

HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc. Details Specifications Features Specifications Main Functions Isolation total RNA (include miRNA)  from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes) Applications RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea Sample amount ≤200 mg Elution volume ≥30μl Time per run 1-24 samples within 30 minutes Liquid carrying volume per column 800µl Binding yield of column 100µg Principle This kit uses glass fiber filter membrane purification technique, and only requires simple combination-washing-elution steps. The sample is lysed and homogenized in the solution containing guanidine salt, ethanol is added to provide appropriate binding conditions, and transferred to the purification column for centrifugation. Up to 100µg of RNA can be selectively bound to the membrane, pollutants are efficiently washed off after three times of washing, and finally the purified RNA is eluted by RNase Free Water. Advantages Completely remove DNA by using of DNase High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration Fast – several samples can be extracted in 40 minutes by column method Sensitive – RNA can be recovered at the level of PG Broad spectrum – various types of plant samples can be processed by diversity of operating procedures Kit Contents Contents R416502 D416503 Purification Times 50 Preps 250 Preps HiPure RNA Mini Columns 50 250 2ml Collection Tubes 100 250 DNase I 600 μl 5 x 600 μl DNase Buffer 6 ml 30 ml Buffer PAL 60 ml 270 ml Buffer GXP2* 20 ml 100 ml Buffer BDP 60 ml 270 ml Buffer RW1 50 ml 250 ml Buffer RW2* 20 ml 2 x 50 ml RNase Free Water 10 ml 30 ml Storage and Stability DNase I should be stored at -20-8°C upon arrival. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.