HiPure Plasmid Mini Kit II

Introduction The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water. Details Specifications Features Specifications Main Functions Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture Applications Enzyme digestion, sequencing, PCR, cloning, etc. Purification method Mini spin column Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type Conventional plasmid, plasmid less than 30KB Sample amount High copy plasmid: 1-5ml culture medium Low copy plasmid : 5-10ml culture medium Yield 20-80µg Elution volume ≥75μl Time per run Complete 1-24 samples in 30 minutes Liquid carrying volume per column 800µl Binding yield of column 70µg Principle The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA). Advantages High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc. Fast – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands) High yield – up to 70µg plasmid can be binded in one column Kit Contents Contents P100202 P100203 Purification Times 50 Preps 250 Preps RNase A 5 mg 20 mg Buffer P1 30 ml 140 ml Buffer P2 30 ml 140 ml Buffer P3 40 ml 200 ml Buffer PW1 30 ml 140 ml Buffer PW2* 12 ml 50 ml Elution Buffer 15 ml 30 ml HiPure DNA Mini Columns III 50 250 ml 2 ml Collection Tubes 50 250 ml Storage and Stability The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C. Purchase Guide Name CAT NO Sample amount Column type Elutio volume Yield Time per run Centrifuge requirements HiPure Plasmid Mini Kit P1001 1-5ml 1.5ml column 30-50μl 5–35µg/5ml ≤30 minutes small centrifuge HiPure Plasmid Mini Kit II P1002 5-15ml 1.5ml column 75-100μl 20-80µg/15ml ≤30 minutes small centrifuge HiPure Plasmid Plus 96 Kit P1006 1-1.5ml 96 well plate 70-150μl 1-15μg/1ml ≤60 minutes 96 well plate centrifuge HiPure Fastfilter Plasmid Midi Kit P1013 30-50ml 15ml column 0.3-0.8ml 50–250µg/50ml ≤60 minutes 15ml centrifuge HiPure Fastfilter Plasmid Maxi Kit P1014 100-200ml 50ml column 0.5-1.5ml 0.4–1mg/200ml ≤60 minutes 50ml centrifuge