HiPure Plasmid Plus 96 Kit

Introduction HiPure Plasmid DNA Plus 96 Kit: Enables up to 96 mini-preps to be performed simultaneously. Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96). Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications. Key Features: Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with the kit is immediately ready for use. Eliminates the need for phenol extraction and ethanol precipitation. High-quality plasmid DNA is eluted in a small volume of Tris buffer or water. Details Specifications Features Specifications Main Functions Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate Applications Enzyme digestion, sequencing, PCR, labeling, etc. Purification method 96 well plate Purification technology Silica technology Process method Manual (centrifugation or vacuum) Sample type Conventional plasmid, plasmid less than 30KB Sample amount 1-1.5ml（x96） Yield 1-15µg/1ml Elution volume ≥70μl Time per run ≤60 minutes Liquid carrying volume per column 800µl Binding yield of column 70µg Principle HiPure Plasmid Procedure: Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps. Procedure Steps: Preparation and Clearing of Bacterial Lysate: The alkaline method is used to prepare and clear bacterial lysate. DNA Binding: Supernatant transferred to a column to bind DNA to the silica membrane. Washing and Elution: Proteins and other impurities washed away. Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA). Advantages High purity – purified plasmid can be directly used in sequencing, enzyme digestion PCR, etc. Fast – it takes only 60 minutes to complete the isolation High yield – up to 1mg plasmid can be bound in one column Economy – high cost-performance Kit Contents Contents P100601 P100602 P100603 Purification Times 1 x 96 Preps 4 x 96 Preps 20 x 96 Preps RNase A 5 mg 20 mg 100 mg Buffer P1 30 ml 120 ml 600 ml Buffer P2 30 ml 120 ml 600 ml Buffer P3 40 ml 180 ml 800 ml Buffer PW1 100 ml 500 ml 2 x 1000 ml Buffer PW2 50 ml 2 x 100 ml 4 x 200 ml Elution Buffer 150 ml 60 ml 300 ml Lysate Clear Plate 1 4 20 HiPure DNA Plate 1 4 20 1.6 ml Collection Plate 1 4 20 0.5ml Elute Plate 1 4 20 Storage and Stability The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C. Purchase Guide Name CAT NO Sample amount Column type Elution volume Yield Time per run Centrifuge requirements HiPure Plasmid Mini Kit P1001 1-5ml 1.5ml column 30-50μl 5–35µg/5ml ≤30 minutes small centrifuge HiPure Plasmid Mini Kit II P1002 5-15ml 1.5ml column 75-100μl 20-80µg/15ml ≤30 minutes small centrifuge HiPure Plasmid Plus 96 Kit P1006 1-1.5ml 96 well plate 70-150μl 1-15μg/1ml ≤60 minutes 96-well plate centrifuge HiPure Fastfilter Plasmid Midi Kit P1013 30-50ml 15ml column 0.3-0.8ml 50–250µg/50ml ≤60 minutes 15ml centrifuge HiPure Fastfilter Plasmid Maxi Kit P1014 100-200ml 50ml column 0.5-1.5ml 0.4–1mg/200ml ≤60 minutes 50ml centrifuge